GreenQuant dsDNA kit contains a fluorescent proprietary DNA-binding dye (488nmEx/522nmEm) that allows accurate and sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in a purified sample.
The assay is highly selective for dsDNA over RNA and provides a linear range between 0.2 and 200 ng of dsDNA input, which corresponds to sample concentrations of 10 pg/μL to 100 ng/μL.
Compared to spectrophotometric DNA quantitation, the assay provides key advantages such as significantly increased sensitivity, high selectivity for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA, and improved contaminant tolerance (protein and carbohydrate molecules).
The kit is ideal for quantifying DNA from low-concentration or precious samples, and for quantifying DNA for use in sensitive applications such as Next-Generation Sequencing (NGS) or digital PCR.
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