Properties for GREEN STAIN 10000X

Description	Bright green fluorescent DNA staining with exceptionally low background for the highest sensitivity
Features	SENSITIVITY: Detection as little as 100 pg of dsDNA per band HIGH CONTRAST: Bright green fluorescence with exceptionally low background SAFE: Very low toxicity EASY TO USE: Substitute your reagent with GREEN STAIN using one of the following protocols: - pre-electrophoresis gel staining for the highest sensitivity - sample pre-staining for the best migration results - post-electrophoresis gel staining for particular protocol needs
Components	10,000X Solution in DMSO
Sufficient for	0,5 mL is sufficient for staining 50 minigels
Storage	-20 °C

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Green Stain 10000X Product Manual

Quick Product Manual GREEN STAIN

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254 nm transillumination: comparison with competitors

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the right (preelectrophoresis gel staining). Gel visualization was done with a standard UV-transilluminator (254 nm), exposure time 1,6 sec.

460 nm epillumination: comparison with competitors

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the right (preelectrophoresis gel staining). Gel visualization was done with GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 se

Comparison with Ethidium Bromide

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the left (preelectrophoresis gel staining). Gel visualization was done with a standard UV-transilluminator (254 nm), exposure time 1,6 sec.

Comparison with GelGreen™

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the left (preelectrophoresis gel staining). Gel visualization was done with a standard UV-transilluminator (254 nm), exposure time 1,6 sec; GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 sec.

Comparison with GelRed™

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the left (preelectrophoresis gel staining). Gel visualization was done with a standard UV-transilluminator (254 nm), exposure time 1,6 sec.

Comparison with SYBR® Green I

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the left (preelectrophoresis gel staining). Gel visualization was done with a standard UV-transilluminator (254 nm), exposure time 1,6 sec; GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 sec.

Comparison with SYBR® Safe

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of the dye indicated on the left (preelectrophoresis gel staining). Gel visualization was done with a standard UV-transilluminator (254 nm), exposure time 1,6 sec; GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 sec.

Detection Limit: Pre Electrophoresis Gel Staining

The nucleic acid-bound GREEN STAIN is efficiently excited at ~254 nm and ~488 nm.

Genomic DNA was electrophoresed on a 1% agarose gel stained with a 1:10000 diluition of GREEN STAIN (pre-electrophoresis gel staining). Gel visualization was done with (A) a standard UV-transilluminator (254 nm), exposure time 1,6 sec; (B) GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 sec.

Detection Limit: Sample Pre Staining

The nucleic acid-bound GREEN STAIN is efficiently excited at ~254 nm and ~488 nm.

Genomic DNA was electrophoresed on a 1% agarose. Samples were stained with GREEN STAIN 500X (sample pre-staining protocol). Gel visualization was done with (A) a standard UV-transilluminator (254 nm), exposure time 1,6 sec; (B) GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 sec.

DNA Ladders: Pre Electrophoresis Gel Staining

A) 30 ng of GeneRuler™ 1 Kb Plus DNA Ladder (Thermo Scientifc) was electrophoresed on a 1% agarose gel at 100 V for 60 minutes.

B) 30 ng of GeneRuler™ 100 bp Plus DNA Ladder (Thermo Scientifc) was electrophoresed on a 2% agarose gel at 100 V for 60 minutes.

C) 30 ng of GeneRuler™ Low range DNA Ladder (Thermo Scientifc) was electrophoresed on a 3% agarose gel at 100 V for 60 minutes.

All the gels were stained with a 1:10000 diluition of GREEN STAIN (pre-electrophoresis gel staining).
Gel visualization was done with GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm flter), exposure time 2 sec.

DNA Ladders: Sample Pre Staining

A) 500 ng of GeneRuler™ 1 Kb Plus DNA Ladder (Thermo Scientifc) was electrophoresed on a 1% agarose gel at 100 V for 60 minutes.

B) 500 ng of GeneRuler™ 100 bp Plus DNA Ladder (Thermo Scientifc) was electrophoresed on a 2% agarose gel at 100 V for 60 minutes.

C) 500 ng of GeneRuler™ Low range DNA Ladder (Thermo Scientifc) was electrophoresed on a 3% agarose gel at 100 V for 60 minutes.

All the samples were stained with GREEN STAIN 1:200 (sample -staining).
Gel visualization was done with GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm flter), exposure time 2 sec.

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