GREEN STAIN – DNA Loading Dye

PRODUCT

GREEN STAIN – DNA Loading Dye

NAGS116

GREEN STAIN – DNA Loading Dye contains a bright green fluorescent dye for DNA stain with a very low toxicity and a pre-mixed loading buffer with tracking dyes (bromophenol blue and xylene cyanol FF) for agarose gels.

GREEN STAIN – DNA Loading Dye allows simultaneous staining of DNA and visual tracking of the migration.

Green Stain – DNA Loading Dye is easy-to-use: just add it to the sample before pipetting it into the gel wells. Gel visualization can be done with a standard transilluminator (254 nm), or with a laser- or LED-based imaging system selecting the SYBR® Green filter (460-490 nm).

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Properties for GREEN STAIN – DNA Loading Dye

Description	Ready-to-use kit to track and stain DNA in agarose gels
Features	Very low toxicity Ready to use kit Optimal signal to background ratio Simple mix with your DNA sample and load into the gel
Applications	DNA staining for agarose gel electrophoresis
Content	Vial A: GREEN STAIN - Dye, Cod. NAGS116,DY Vial B: GREEN STAIN - Loading buffer, Cod. NAGS116,LB
Shipping Condition	Refrigerated
Storage	Store at -20 °C; after reconstitution, store at +4 °C for 6 months

Online Resources & Supporting Documents

FILTERS Show all CoC SDS Product Manual Technical Note

CoC MF26A-XP NAGS116 Green Stain DNA Loading Dye

CoC OB25B-XP NAGS116 Green Stain Loading Dye

CoC OF09D-XP NAGS116 Green Stain DNA Loading Dye

CoC OG16A-XP NAGS116 Green Stain DNA Loading Dye Rev01

CoC RH08A-XP NAGS116 Green Stain DNA Loading Dye Rev00

IT SDS GREEN STAIN Loading Dye NAGS116 Rev01

EN SDS GREEN STAIN Loading Dye NAGS116 Rev01

Green Stain DNA Loading Dye Product Manual

Quick Product Manual GREEN STAIN

GreenQuant dsDNA kit Technical Note

Gallery

DNA Ladders And Genomic DNA GREEN STAIN DNA Loading Dye

A) Genomic DNA.

B) 200 ng of GeneRuler™ 100 bp Plus DNA Ladder (Thermo Scientific).

C) 200 ng of GeneRuler™ 1 Kb Plus DNA Ladder (Thermo Scientific).

All the samples were stained with GREEN STAIN - DNA Loading Dye and electrophoresed on a 1% agarose gel at 100 V for 60 minutes. Gel visualization was done with GE Healthcare "ImageQuant ™ LAS 4000" (460 nm filter), exposition time 2 sec.

Green Stain DNA Loading Dye allows track and stain of DNA on agarose gel

Green Stain DNA Loading Dye allows track and stain of DNA on agarose gel
Green Stain-DNA Loading Dye is easy to use: simply mix 2 µL of Green Stain-DNA Loading Dye to 2-10 µL of your DNA sample and molecular marker before pipetting into the gel wells. It is not required to add any stain to the gel or running buffer.

Comparison with Midori Direct

Five microliters of genomic DNA and DNA Ladder were mixed with 2 µL of Green Stain DNA Loading Dye or Midori Direct and electrophoresed on a 1% agarose gel. Gel visualization was done with GE Healthcare Life Sciences "ImageQuant LAS 4000" (460 nm), exposure time 2 sec.