Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR-ONE EXTREME and its competitors
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 2, 5 μg to 0.039 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:10000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1: 200000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 120 seconds