WESTAR-ONE EXTREME — Ultra-Sensitive, Ready-to-Use Low-Femtogram ECL Substrate

PRODUCT

WESTAR-ONE EXTREME — Ultra-Sensitive, Ready-to-Use Low-Femtogram ECL Substrate

XLSU180

What is WESTAR-ONE EXTREME?

WESTAR-ONE EXTREME is a patented, next-generation chemiluminescent substrate specifically developed for researchers who demand the highest levels of sensitivity in Western blot detection. Featuring an innovative single-solution formulation, it delivers low-femtogram detection sensitivity, making it the most sensitive ready-to-use ECL substrate in its category.

Designed to simplify workflows while maximizing analytical performance, WESTAR-ONE EXTREME eliminates the need for reagent mixing, reducing preparation time and minimizing potential experimental variability. Bring the reagent to room temperature, and it is ready for use.

The superior signal intensity generated by WESTAR-ONE EXTREME enables the detection of extremely low protein concentrations that may remain undetectable with conventional chemiluminescent substrates, providing greater confidence when working with low-abundance proteins, limited sample quantities, or challenging biological targets.

Benchmarked against: Benchmarked against Immobilon® Forte under identical experimental conditions.

 

Key Performance Data at a Glance

 

Why Choose WESTAR-ONE EXTREME

 

Exceptional Sensitivity for Challenging Targets

Under identical experimental conditions, WESTAR-ONE EXTREME demonstrated superior detection performance compared with Immobilon® Forte, revealing six detectable bands versus three and highlighting its remarkable sensitivity and signal-generation capability.

 

High Performance with Lower Antibody Consumption

The substrate’s outstanding efficiency enables the use of significantly higher antibody dilutions while maintaining strong signal intensity, resulting in lower reagent consumption, reduced experimental costs, and improved laboratory productivity.

 

Patented One-Solution Technology

WESTAR-ONE EXTREME’s patented one-solution technology eliminates reagent mixing, reducing hands-on time and workflow complexity while maximizing convenience and reproducibility.

 

Ready-to-Use After Reaching Room Temperature

Bring WESTAR-ONE EXTREME to room temperature, and it is ready for use — no additional preparation steps, reducing experimental variability.

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Properties for WESTAR-ONE EXTREME — Ultra-Sensitive, Ready-to-Use Low-Femtogram ECL Substrate

Features Low-femtogram detection
No mixing is required
Low antibody consumption to save your money
Ready-to-use after reaching the RT
Substrate type HRP (Horseradish Peroxidase) Substrate
Components Luminol derivative/enhancer/peroxide solution
Antibody Dilution Range Primary: 1:5000 - 1:100000
Secondary: 1:100000 - 1:500000
(from 1 mg/mL stock solution)
Available as 250 mL kit (2,500 cm2 of membrane)
Signal duration 2 hours
Storage Stable for 1 year at 2-8°C
Shipping RT

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References for WESTAR-ONE EXTREME — Ultra-Sensitive, Ready-to-Use Low-Femtogram ECL Substrate

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WESTAR-ONE EXTREME delivers low-femtogram detection sensitivity, making it the most sensitive ready-to-use ECL substrate in its category.

Under identical experimental conditions, WESTAR-ONE EXTREME revealed six detectable bands compared with three for Immobilon® Forte, demonstrating superior sensitivity and signal generation.

No. WESTAR-ONE EXTREME uses a patented one-solution technology —bring the reagent to room temperature, and it is ready for use, with no mixing required.

Yes. Its outstanding efficiency allows the use of significantly higher antibody dilutions while maintaining strong signal intensity, reducing reagent consumption and experimental costs.

The signal lasts up to 2 hours, providing ample time for image acquisition and exposure optimization.

Primary antibody: 1:5,000–1:100,000. Secondary antibody: 1:100,000–1:500,000 (from a 1 mg/mL stock solution).

It is shipped at room temperature and is stable for 1 year when stored at 2–8°C.

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