WESTAR-ONE BASIC is our entry-level pre-mixed chemiluminescent HRP substrate for mid-picogram detection level in Western Blot.
Ideal for routinely analysis. The formulation provides a low background for a high signal to noise ratio.
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WESTAR-ONE BASIC
PRODUCT
WESTAR-ONE BASIC
XLSU177
Properties for WESTAR-ONE BASIC
| Features | Mid-picogram detection No mixing is required Ideal for everyday detection needs Ready-to-use at RT |
| Substrate type | HRP (Horseradish Peroxidase) Substrate |
| Components | Luminol derivative/enhancer/peroxide solution |
| Antibody Dilution Range | Primary: 1:100 - 1:5000 Secondary: 1:1000 - 1:20000 (from 1 mg/mL stock solution) |
| Available as | 250 mL (2,500 cm2 of membrane) |
| Signal duration | 3 hours |
| Storage | Stable for 1 year at RT |
| Shipping | RT |
Online Resources & Supporting Documents
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Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR-ONE BASIC and its competitors
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1: 20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare.
Exposure time: 120 seconds
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1: 20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare.
Exposure time: 120 seconds
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