WESTAR HYPERNOVA

PRODUCT

WESTAR HYPERNOVA

XLS149

WESTAR HYPERNOVA is the brightest ECL substrate ever seen on the market.

Its extreme sensitivity and extraordinary light output allow the detection of trace amounts of proteins.

The formulation provides a low background for an extremely high signal to noise ratio.

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Properties for WESTAR HYPERNOVA

Description	WESTAR HYPERNOVA is the brightest ECL substrate ever seen on the market. Its extreme sensitivity and extraordinary light output allow the detection of trace amounts of proteins. The formulation provides a low background for an extremely high signal to noise ratio.
Features	Detection of trace amounts of proteins Extreme significance of the experiment Shortened exposure time Reduced consumption of antibodies and precious samples
Components	Luminol derivative/enhancer solution (A) Peroxide solution (B)
Antibody Dilution Range	Primary 1:10.000-1:200.000 Secondary 1:300.000-1:1.000.000 (from 1 mg/mL stock solution)
Available as	2 x 10 mL kit (200 cm2 of membrane) 2 x 50 mL kit (1000 cm2 of membrane)
Signal duration	5 hours
Storage	Stable for 1 year at RT Product is shipped at ambient temperature.

Online Resources & Supporting Documents

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MSDS XLS149L Westar Hypernova EN

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WESTAR And WESTAR ONE Product Manual

Quick Product Manual WESTAR

WESTAR HYPERNOVA Technical Note

HYPERNOVA ECL Substrate for WB and ELISA RevGG20231003

Gallery

Enhanced precision in Western blotting with WESTAR HYPERNOVA

A) Purified IKBα detection with WESTAR HYPERNOVA. Triplicate blots containing 1.5-fold dilutions of purified IKBα were incubated with primary antibody (Rabbit anti- IKBα) 1:1000 and secondary antibody (Goat anti Rabbit-HRP) 1: 500000 and imaged for 10 seconds with ImageQuantTM LAS 4000 (GE Healthcare).
B) Integrated signal intensity. Data show that WESTAR HYPERNOVA delivers a linear signal response, over a wide range of protein levels.

WESTAR HYPERNOVA Graph Comparison with Competitors

Western blotting detection of purified IKB α
Purified IKB α 1,5 -fold dilutions from 1000 to 5,1 pg
Ab 1° Rabbit anti IKB α 1:1000
Ab 2° Goat anti rabbit 1:500000
Exposure time: 10 seconds
Imager: LAS4000 (GEHC)

Enhanced sensitivity of WESTAR HYPERNOVA compared to one of the current top-level substrates (SuperSignalTMWest Femto-Thermo ScientificTM).

A) Purified IKBα detection with either WESTAR HYPERNOVA or SuperSignalTM West Femto. Triplicate blots for each substrate containing 1.5-fold dilutions of purified IKBα from 667 pg to 5 pg were simultaneously imaged for 10 seconds with ImageQuantTM LAS 4000 (GE Healthcare).
B) Signal-to-noise ratio (S/N) analysis. The inset enlargement shows the enhanced sensitivity of WESTAR HYPERNOVA.
(LOD = 5 pg) compared to SuperSignalTM West Femto (LOD = 39 pg).
(LOD = Limit of Detection)

WESTAR HYPERNOVA enables to save protein samples

Triplicate blots for each substrate containing serial dilutions of purified IKBα were incubated with primary antibody (Rabbit-anti IKBα) 1:1000 and secondary antibody (Goat-anti Rabbit-HRP) 1:500000 and were simultaneously imaged for 10 seconds. Bar
graph shows that HYPERNOVA produces the same signal intensity as AmershamTM ECL SelectTM with up to 8-times less protein.

WESTAR HYPERNOVA enables to save expensive primary antibodies

Triplicate blots containing serial dilutions of purified IKBα were probed with 1:5000 and 1:50000 primary antibody (Rabbit anti- IKBα) dilutions and with 1:500000 secondary antibody
(Goat anti Rabbit-HRP) dilutions, and detected with either WESTAR HYPERNOVA - Cyanagen or Clarity Max™ - Bio-Rad. Blots were imaged simultaneously with 60 seconds exposure time.

Signal-to-noise ratio comparison between HYPERNOVA and a top-level substrate.

WESTAR HYPERNOVA produces a comparable result to Clarity Max™- Bio-Rad with 10-times less primary antibody.

Extremely high sensitivity detection with extra-short exposure time

Triplicate blots containing 1.5-fold dilutions of purified IKBα from 667 pg to 5 pg were probed with 1:1000 primary antibody dilutions and with 1:500000 secondary antibody (Goat anti Rabbit-HRP) dilutions and detected with either WESTAR HYPERNOVA - Cyanagen or Clarity Max™- Bio-Rad. Blots were imaged simultaneously with 10-60-120-300 seconds exposure time.

Extremely reduced variability using WESTAR HYPERNOVA

200 µL of WESTAR HYPERNOVA were added to 16 wells of a 96-well black plate, furthermore adding HRP enzyme at a final concentration of 0.8 ng/mL and reading with Victor3 microplate reader (Perkin Elmer).

Western blotting detection of purified IKBα

Purified IKB α 1,5 -fold dilutions from 1000 to 5,1 pg
Ab 1° Rabbit anti IKB α 1:1000
Ab 2° Goat anti rabbit 1:500000
Exposure time: 10 seconds
Imager: LAS4000 (GEHC)

Increased reproducibility in Western blotting with WESTAR HYPERNOVA

Standard deviation (ST.DEV) and coefficient of variation (CV%) were calculated on the mean of signal intensities from membranes M1, M2, M3.

Reviews

The researcher: «WESTAR HYPERNOVA is more sensitive than ECL SELECT from GE Healthcare»

IRCSS Mondino Foundation, Pavia, Italy

The researcher: «I obtained a good result with WESTAR HYPERNOVA. Sample was too faint to be detected with Clarity Max from BIO-RAD».

Department of Sciences and Biological Technologies. University of Salento- Italy

The researcher: «Significantly higher sensitivity for samples with extremely low antigen content»

Ludwig Maximilian University Munich- Germany.

The researcher: «Excellent sensitivity compared to the substrate used in our lab»

Telethon Institute of Genetics and Medicine. Naples- Italy.

The researcher: «We observed that WESTAR Hypernova has a detection time shorter than WESTAR Supernova. We also observed that the signal found was stronger than that one of Supernova. ».

Department of Biomolecular and Pharmacological Sciences. University of Milan- Italy