WESTAR ETA C ULTRA 2.0 is an ECL substrate with stable light output for mid to low femtogram detection level.
The perfect ECL substrate for applications requiring very high signal intensity and a long signal duration.
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WESTAR ETA C ULTRA 2.0
PRODUCT
WESTAR ETA C ULTRA 2.0
XLS075
Properties for WESTAR ETA C ULTRA 2.0
| Description | WESTAR ETA C ULTRA 2.0 is an ECL substrate with stable light output for mid to low femtogram detection level. The perfect ECL substrate for applications requiring very high signal intensity and a long signal duration. |
| Features | Mid to low femtogram detection The best compromise between high signal intensity and a very long signal duration A value-priced top performer Working solution stable for at least three days |
| Components | Luminol/enhancer solution (A) Peroxide solution (B) |
| Antibody Dilution Range | Primary: 1:5.000 – 1:50.000 Secondary: 1:50.000 - 1:250.000 (from 1 mg/mL stock solution) |
| Available as | 2 x 50 mL kit (1000 cm2 of membrane) |
| Signal duration | > 24 hours |
| Storage | Stable for 1 year at RT. Product is shipped at ambient temperature |
Online Resources & Supporting Documents
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Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR ETA C ULTRA 2.0 and its competitors.
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5μg to 0,078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:7500
Secondary antibody: Goat anti-Rabbit IgG HRP (2mg/mL) (abcam®) 1:100000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:7500
Secondary antibody: Goat anti-Rabbit IgG HRP (2mg/mL) (abcam®) 1:100000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Extended signal duration with WESTAR ETA C ULTRA 2.0.
For either WESTAR ETA C ULTRA 2.0 or its competitors, exposure time is 180 seconds for each time points (0-2-5-8-11-20 hours).
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