What is WESTAR ANTARES?

WESTAR ANTARES is a chemiluminescent (ECL) substrate for Western blot detection at the mid-femtogram level, combining high sensitivity with one of the longest signal durations in its class — up to 20 hours. It is a drop-in replacement for the most widely used substrates on the market, requiring no protocol changes.

Direct alternative to: Amersham™ ECL Prime™ (GE Healthcare), Clarity™ (Bio-Rad), and SuperSignal™ West Dura and West PICO PLUS (Thermo Scientific™).

Key Performance Data at a Glance

Why Choose WESTAR ANTARES

Benchmark-Level Sensitivity for Routine Western Blotting

WESTAR ANTARES delivers stable, mid-level performance ideal for everyday experiments, with sensitivity and signal intensity that outperform Amersham™ ECL Prime™ (GE Healthcare), Clarity™ (Bio-Rad), and SuperSignal™ West Dura and West PICO PLUS (Thermo Scientific™) in routine applications — while being a seamless, no-protocol-change substitute for all major ECL substrates.

Wide Linear Dynamic Range for Accurate Quantification

Reliable quantitative Western blot data depends on staying within the linear dynamic range, where the chemiluminescent signal is directly proportional to protein concentration. WESTAR ANTARES achieves an R² of 0.998, with a strong signal and very low background — producing a high signal-to-noise ratio that is comparable to ECL Prime™ and SuperSignal™ West Dura, and significantly better than Clarity™ and SuperSignal™ West PICO PLUS. This wide range allows accurate quantification of both low- and high-abundance proteins on the same blot, in a single exposure.

Top Signal Duration in Its Class

In a comparative study tracking HDAC-1 signal intensity over time (2.5 µg total protein, up to 20 hours), WESTAR ANTARES retained about 50% of its initial signal after 5 hours, while most competing substrates dropped below 10%. The signal remained detectable for up to 20 hours of incubation. This extended window supports repeated exposures without reapplying the substrate, processing multiple blots within a single experimental run, and long-term CCD camera exposures for detecting faint, low-abundance bands.

High Reproducibility, Fewer Artefacts

Across triplicate blots of the same experiment, WESTAR ANTARES shows less than 15% variability, remaining robust against common sources of error — sample loading, transfer efficiency, blocking, and antibody performance. The result: more significant, more reproducible experimental data with a lower risk of artifacts.