WESTAR ANTARES is an ECL substrate with stable light output for mid femtogram detection level.
The perfect ECL formulation combining great sensitivity and long signal duration.
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WESTAR ANTARES
PRODUCT
WESTAR ANTARES
XLS142
Properties for WESTAR ANTARES
| Description | WESTAR ANTARES is an ECL substrate with stable light output for mid femtogram detection level. The perfect ECL formulation combining great sensitivity and long signal duration. |
| Features | Mid femtogram detection Extended signal duration Working solution stable for at least three days High range flexibility |
| Components | Luminol/enhancer solution (A) Peroxide solution (B) |
| Antibody Dilution Range | Primary: 1:1.000 - 1:15.000 Secondary: 1:25.000 - 1:150.000 (from 1 mg/mL stock solution) |
| Available as | 2 x 125 mL kit (2500 cm2 of membrane) |
| Signal duration | > 24 hours |
| Storage | Stable for 1 year at RT Product is shipped at ambient temperature |
Online Resources & Supporting Documents
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Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR ANTARES and its competitors.
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:5000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:75000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:5000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:75000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Extended signal duration of WESTAR ANTARES.
For either WESTAR ANTARES or its competitors, exposure time is 180 seconds for each time points (0-2-5-8-11-20 hours).
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