SIA is a heterobifunctional amine to sulfhydryl crosslinking reagent. SIA contains one NHS ester group and a iodoacetyl reactive group at each end of a short 1.5 angstroms spacer. The NHS ester groups readily react towards primary amines at pH 7-9.
The iodoacetyl group reacts towards sulfhydryl (or thiol) groups at pH > 7.5.
SIA is not water soluble and must be dissolved in an organic solvent such as DMSO or DMF prior to use.
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Properties for SIA Crosslinker Reagent
| Features | Water-insoluble and non cleavable |
| Name | N-hydroxysuccinimidyl iodoacetate |
| Linear formula | C6H6INO4 |
| Molecular weight | 283.02 g/mol |
| Melting point | 144-145 °C |
| Storage | Desiccated at -20 °C under Argon. Product is shipped at ambient temperature |
| Assay | ≥ 97% |
| Identity (NMR) | Complies |
| Solubility | DMSO, DMF. Water insoluble |
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References for SIA Crosslinker Reagent
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