RUBY Gel Stain

PRODUCT

RUBY Gel Stain

PRTD066

RUBY Gel Stain is a ready to use kit for rapid and sensitive protein staining in 1D and 2D SDS polyacrylamide electrophoresis gels.

It enables optimal visualization and quantitation of proteins with high contrast and the same sensitivity as silver staining without its drawbacks.

The staining procedure is a simple 220 minutes, three step protocol.After staining, proteins can be removed from the gel and analyzed by mass spectrometry without interference from the stain.

The dye has optimal excitation at 302 and 470 nm, with an emission maximum at approximately 610 nm.RUBY GEL STAIN can be excited with UV-light, UV transilluminator and 405, 445, 473-488 nm laser sources.

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Properties for RUBY Gel Stain

Features	Ready to use kit High purity dye: >98% Optimal signal to background ratio Strong, uniform and reproducible signal from 0.2 ng to 10 ng protein Fast staining protocol (220 min) Convenient: fixing and destaining solutions included in the kit The gel can be visualized using excitation wavelength in the 400-500 nm range with bright emission peak centred around 610 nm.
Components	Solution A 50 mL, solution B 100 mL Solution A 250 mL, solution B 500 mL
Sufficient for	Solution A 50 mL, solution B 100 mL for 1 minigel (8,6 x 6,7 x 0,1 cm) Solution A 250 mL, solution B 500 mL for 5 minigel (8,6 x 6,7 x 0,1 cm)
Storage	RT

Online Resources & Supporting Documents

FILTERS Show all CoC MSDS Product Manual

CoC IC15A-LB PRTD066 Ruby Gel Stain

MSDS PRTD066A Ruby Gel Stain EN

MSDS PRTD066B Ruby Gel Stain EN

Quick Product Manual RUBY GEL STAIN EN

Ruby Gel Stain Product Manual

Gallery

RUBY Gel Stain on 1D and 2D gel

a) Ruby Gel Stain on a 1D gel. Two-fold dilution series of Precision Plus Protein™ Unstained BIO-RAD marker separated on a BIO-RAD Mini-PROTEAN™ TGX™ 12% and stained following Ruby Gel Stain 220’ min protocol. Images acquired by GEHC ImageQuant™ LAS4000, Exc 312 nm, exposure time 1s. Lane 9 is approximately 0.4 ng b) Ruby Gel Stain on a 2D gel. 60 µg of Bio-Rad ReadyPrep™E.coli Protein Sample, separated on Bio-Rad ReadyPrep™ IPG strips pH 3-10 NL and then on a SDS-PAGE (T%8-16) with a Bio-Rad PROTEAN™ II XI system. The gel was stained following Ruby Gel Stain 220’ min protocol.
The image was digitalized by Bio-Rad Molecular Imager FX™Pro fluorescent imager, PMT Voltage Medium Sample Intensity.