PCNA

PRODUCT

PCNA

ABP141

Proliferating cell nuclear antigen, PCNA, is a cofactor of DNA polymerase delta, and is expressed in the nuclei of cells during the DNA synthesis phase (S phase) of the cell cycle.

PCNA is involved in eukaryotic DNA replication. PCNA is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, S. pombe, S. cerevisiae, K. lactis, E. gossypii, M. grisea, N. crassa, A. thaliana, and rice.

Human PCNA with 261 amino acids share 97% identity with rodent homologs and 36% identity with yeast homologs. PCNA is not a good loading control for non-proliferating cells or cells with anti-proliferation treatment.

Properties for PCNA

Features Tool for normalizing gel loading differences and for Western blot quantification
Sensitive and reproducible 
Host Species Rabbit
Applications WB, IHC, IF, IP
Species reactivity Hu Ms Rt
Concentration 1 mg/mL
MW (kDa) 29 kDa
Dilution Western Blot dilution  1:500-1:2.000
Immunohistochemistry dilution  1:50-1:200
Immunofluorescence dilution  1:20-1:50
Immunoprecipitation dilution  1:20-1:50
Shipping Condition Room Temperature
Storage Store at -20°C for long-term storage (recommended)
Store at +4°C for short-term (1-2 weeks)
Avoid freeze/thaw cycle

Online Resources & Supporting Documents

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References for PCNA

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