MBS, is a heterobifunctional amine to sulfhydryl crosslinking reagent. MBS contains one NHS ester group and a maleimide reactive group at each end of a small aromatic spacer. The sulfo-NHS ester groups readily react towards primary amines at pH 7-9.
The maleimide group reacts towards sulfhydryl (or thiol) groups at pH 6.5-7.5.
MBS is not water soluble and must be dissolved in an organic solvent such as DMSO or DMF prior to use.
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Properties for MBS Crosslinker Reagent
| Features | Water-insoluble and non cleavable |
| Name | 3-Maleimidobenzoic acid N-hydroxysuccinimide ester, m-maleimidobenzoyl-N-hydroxysuccinimide ester |
| Linear formula | C15H10N2O6 |
| Molecular weight | 314.30 g/mol |
| Melting point | 175-177 °C |
| Storage | Desiccated at -20 °C under Argon. Product is shipped at ambient temperature |
| Assay | ≥ 97% |
| Identity (NMR) | Complies |
| Solubility | DMSO, DMF. Water insoluble |
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References for MBS Crosslinker Reagent
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