Nuclear lamins are fibrous proteins providing structural function and transcriptional regulation in the cell nucleus. Nuclear lamins interact with membrane-associated proteins to form the nuclear lamina on the interior of the nuclear envelope.

Among the lamins, LAMIN B1 tends to be used as a loading control. Lamin B1 is conserved across species. Human Lamin B1 shares 95% identity with rodent homologs, and 36% identity with the fruit fly counterpart. Human Lamin B1 is more than 50% identical to other human lamins. Lamin B1 protein is not suitable as a loading control for samples without nuclear envelope.

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Properties for LAMIN B1

Features Tool for normalizing gel loading differences and for Western blot quantification 
Sensitive and reproducible 
Host Species Rabbit
Applications WB, IHC, ELISA
Species reactivity Hu Ms Rt
Concentration 1 mg/mL
MW (kDa) 67 kDa
Dilution Western Blot dilution  1:500-1:2.000
Immunohistochemistry dilution  1:100-1:300
ELISA dilution  1:40.000
Shipping Condition Room Temperature
Storage Store at -20°C for long-term storage (recommended)
Store at +4°C for short-term (1-2 weeks)
Avoid freeze/thaw cycle

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