IR-BLOT 700 Goat anti-Rabbit ensures extremely low background and specific signal for high-quality quantitative Western Blotting.
Combined with IR-BLOT 800 Goat anti-Mouse, it allows measuring multiple targets in a single experiment.
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IR-BLOT 700 Goat anti-Rabbit
PRODUCT
IR-BLOT 700 Goat anti-Rabbit
F2H111
Properties for IR-BLOT 700 Goat anti-Rabbit
| Description | Highly cross adsorbed Goat anti-Rabbit IgG (H&L) - IR-BLOT 700, conjugated secondary antibody |
| Features | Highly cross-adsorbed antibodies High sensitivity and low background Quantitative Western Blotting Optimized for multicolor IR-Western blotting |
| Cross Adsorbed Species | Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins |
| Exc-Em (nm) | 678-690 nm (abs-em) |
| Applications | Infrared quantitative Western blotting |
| Content | 0.1 mg of fluorescent secondary antibody lyophilized in phosphate buffer saline, pH 7.4. Contains 10 mg/mL BSA as stabilizer and 0.01% sodium azide as preservative, after reconstitution |
| Shipping Condition | Room Temperature |
| Storage | Protect from light. Store at +4 °C prior to reconstitution. Once reconstituted, stable for up to 6 months at +4 °C |
Online Resources & Supporting Documents
FILTERS
Gallery
Multiplex fluorescent western blot for the detection of Vinculin and HSP-90 on HeLa whole cell lysates with Cyanagen IR-BLOT Secondary antibodies
Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein.
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS.
Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 and Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody:IR-BLOT 700 Goat anti-Rabbit (red) and IR-BLOT 800 Goat anti- Mouse (green)1:25000.
Imager: ODISSEY ® CLX – LI-COR.
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS.
Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 and Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody:IR-BLOT 700 Goat anti-Rabbit (red) and IR-BLOT 800 Goat anti- Mouse (green)1:25000.
Imager: ODISSEY ® CLX – LI-COR.
Fluorescent Western blot with Cyanagen IR-BLOT and LI-COR IRDye® Secondary antibodies
Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein.
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS.
Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Rabbit (Cyanagen) and IRDye® 680RD Goat anti-Rabbit (LI-COR) 1:25000.
Imager: ODISSEY ® CLX – LI-COR.
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS.
Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Rabbit (Cyanagen) and IRDye® 680RD Goat anti-Rabbit (LI-COR) 1:25000.
Imager: ODISSEY ® CLX – LI-COR.
References for IR-BLOT 700 Goat anti-Rabbit
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