GMBS is a heterobifunctional amine to sulfhydryl crosslinking reagent. GMBS contains one NHS ester group and a maleimide reactive group at each end of an aliphatic 5-atom spacer. The NHS ester groups readily react towards primary amines at pH 7-9.
The maleimide group reacts towards sulfhydryl (or thiol) groups at pH 6.5-7.5.
GMBS is not water soluble and must be dissolved in an organic solvent such as DMSO or DMF prior to use.
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