GMBS is a heterobifunctional amine to sulfhydryl crosslinking reagent. GMBS contains one NHS ester group and a maleimide reactive group at each end of an aliphatic 5-atom spacer. The NHS ester groups readily react towards primary amines at pH 7-9.
The maleimide group reacts towards sulfhydryl (or thiol) groups at pH 6.5-7.5.
GMBS is not water soluble and must be dissolved in an organic solvent such as DMSO or DMF prior to use.
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Properties for GMBS Crosslinker Reagent
| Features | Water-insoluble and non cleavable |
| Name | N-Succinimidyl 4-maleimidobutyrate,4-Maleimidobutyric acid N-succinimidyl ester, N-(γ-maleimidobutyryloxy)-N-hydroxysuccinimide ester |
| Linear formula | C12H12N2O6 |
| Molecular weight | 280.20 g/mol |
| Melting point | 123-125 °C |
| Storage | Desiccated at -20 °C under Argon. Product is shipped at ambient temperature |
| Assay | ≥ 97% |
| Identity (NMR) | Complies |
| Solubility | DMSO, DMF. Water insoluble |
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References for GMBS Crosslinker Reagent
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