BS3 (Sulfo-DSS) is a homobifunctional amine-specific crosslinking reagent based on NHS-ester reactive groups for selective conjugation of primary amines.
BS3 contains two sulfo-NHS ester groups at both ends of a 8-carbon spacer.
The sulfo moieties provide excellent water solubility and allows BS3 to be used in acqueous buffer solutions without the need of organic solvents that may cause protein perturbation.
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Properties for BS3 Crosslinker Reagent
| Features | Water-soluble, non-cleavable and membrane impermeable Readily reacts with primary amines in the pH range 7-9 |
| Name | Bis(sulfosuccinimidyl) suberate sodium salt, Suberic acid bis(3-sulfo-N-hydroxysuccinimide ester) sodium salt |
| Linear formula | C16H18N2Na2O14S2 |
| Molecular weight | 572.43 g/mol |
| Melting point | 138-139 °C |
| Storage | Desiccated at -20 °C under Argon. Product is shipped at ambient temperature |
| Assay | ≥ 97% |
| Identity (NMR) | Complies |
| Solubility | Soluble in cold and hot water |
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References for BS3 Crosslinker Reagent
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