VIV-ON Fixable Viability Dyes for Flow Cytometry

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VIV-ON Fixable Viability Dyes for Flow Cytometry

Excluding dead cells from data is recommended for all staining protocols to ensure experimental accuracy. VIV-ON fixable viability dyes allow accurate discrimination between live and dead cells in flow cytometry analysis, based on cell membrane integrity. VIV-ON preserve the staining pattern after fixation and/or permeabilization.

VIV-ON fixable viability dyes are amine reactive and cell impermeable. Dead cells with compromised cell membranes are typically labelled to a higher extend due to reaction with intracellular amines resulting in highly fluorescent dead cells. Impermeable live cells, on the contrary, are labelled only on the cell surface and show dim fluorescence

Features

  • High brightness for optimal differentiation between live and dead cells
  • Dry formulation to guarantee high stability
  • Unlike 7-AAD and PI, labelled cells can be fixed, permeabilized, washed and stained
  • Suitable for any cell species

Applications

Cell Analysis Flow Citometry Imaging

Products collected in this line

PRODUCT NAME FEATURES CAT: NR
VIV-ON Violet 500 FVD High brightness for optimal differentiation between live and dead cells
Ready-to-use kit, DMSO pre-diluted to test size formulation
Suitable for any cell species
Ideal for 405 nm laser excitation

405-510 nm (abs-em)
FVA126N
VIV-ON Blue 520 FVD High brightness for optimal differentiation between live and dead cells
Ready-to-use kit, DMSO pre-diluted to test size formulation
Suitable for any cell species
Ideal for 488 nm laser excitation

488-520 nm (abs-em)
FVA127N
VIV-ON Red 660 FVD High brightness for optimal differentiation between live and dead cells
Ready-to-use kit, DMSO pre-diluted to test size formulation
Suitable for any cell species
Ideal for 633 nm laser excitation

633-660 nm (abs-em)
FVH128N
VIV-ON Red 780 FVD High brightness for optimal differentiation between live and dead cells
Ready-to-use kit, DMSO pre-diluted to test size formulation
Suitable for any cell species
Ideal for 633 nm laser excitation

633-780 nm (abs-em)
FVL129N

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