CROSSLINKING REAGENTS are chemical compounds used to covalently bind, or conjugate, biomolecules together. Cyanagen’s crosslinker reagents are classified according to the reactive groups, their hydrophobicity or hydrophilicity, the length of the spacer between the reactive groups. They can be homobifunctional or heterobifunctional, membrane permeable or impermeable. The spacer arm can also be cleavable.
Longer spacer arms are usually more effective for coupling larger proteins or those where the reactive side-chains are sterically protected.