Western Blotting Troubleshooting – CYANAGEN TIPS – Uneven Bands

Sometimes, at the end of a Western blot, uneven bands appear on the membrane.
Several factors may cause these bands.
We provide hints to fix each root cause.

• Uneven gel run. Load all available wells. Empty wells can be loaded with sample buffer.
• Voltage or current was too high during electrophoresis. Reduce voltage or current during electrophoresis.
• Effects of high salt in samples. Reduce NaCl concentration in TBS-T Buffer (100mM÷350mM).

Cyanagen developed three ECL substrate lines:

WESTAR (ECL two-solution format), for more details click here
WESTAR-ONE (ECL one-solution format), for more details click here
WESTAR-TAB (ECL tablet format), for more details click here

REQUESTS for samples: sales@cyanagen.com