WESTAR-ONE Extreme, the most sensitive ECL substrate for Western blotting in a single solution format

WESTAR-ONE Extreme, with detection in the low-femtogram levels, is the most sensitive chemiluminescent ECL substrate for Western blotting assays in a single solution.

Its excellent signal intensity allows the detection of very low amounts of proteins, reducing the quantity of antibodies needed.

This feature is possible due to a patented technology (Figure 1).

Figure 1. Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR-ONE EXTREME and its competitors.
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 2,5 μg to 0.039 μg of total protein
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:10000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:200000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare). Exposure time: 120 seconds

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