STAR FLUOR Secondary Antibodies
STAR FLUOR Secondary Antibodies
for Fluorescent Immunoassays
Star Fluor secondary antibodies are high-quality secondary antibodies designed for use in a variety of immunoassays including cell imaging, flow cytometry and fluorescent western blotting.
These antibodies are highly cross-adsorbed, ensuring minimal cross-reactivity when using antibodies from different host species. The superior brightness allows the detection of a very low amount of proteins with a high signal-to-noise ratio.
Features
• Superior brightness for more sensitive detection
• Highly cross-adsorbed antibodies for lower cross-reactivity
• Low background for higher signal-to-noise ratio
• Stable signal for extended and repeated exposures
* For western blotting in the infrared region we recommend IR-BLOT 700 and IR-BLOT 800 secondary antibodies.
Applications
Western Blotting
The use of fluorescence in immunoassays allows a fast, reproducible and quantitative detection of the analyte.
Fluorescent detection in western blotting offers the following advantages:
• Stable signal for enhanced reproducibility and accuracy in protein quantitation. Blots can be stored and re-imaged after months without alteration in signal intensity
• Wider dynamic range
• Multiplexing – testing multiple targets on the same blot, at the same time
Fluorescent western blotting detection of Human Transferrin with Rabbit anti-human transferrin primary antibody and Star Fluor 550 Goat anti-Rabbit (red), Star Fluor 645 Goat anti-Rabbit (green). Imager: ImageQUANTTM LAS 4000 GEHC.
Cell Imaging
Star Fluor secondary antibodies are the perfect choice for cell imaging, offering an outstanding performance:
• Superior brightness for the detection of low expressed proteins with high sensitivity
• Excellent photostability for long and repeated exposure
Secondary antibodies conjugated with dyes in the infrared region (Star Fluor 680 and Star Fluor 770) allow reduced scattering and enhanced tissue penetration depth.
(A) Immunohistochemical staining on mouse hippocampal neurons of MAP-2 (blue) using Anti-MAP-2 Primary Antibody and Star Fluor 645 Goat anti-Rabbit and betaIII tubulin (green) using Anti- betaIII tubulin Primary Antibody and Star Fluor 488 Goat anti-Mouse.
(B) Immunohistochemical staining on mouse hippocampal neurons of MAP-2 (red) using Anti-MAP-2 Primary Antibody and Star Fluor 550 Goat anti-Rabbit and betaIII tubulin (green) using Anti- betaIII tubulin Primary Antibody and Star Fluor 488 Goat anti-Mouse.
(C) Immunohistochemical staining on mouse glia of GFAP (red) using Anti-GFAP Primary Antibody and Star Fluor 550 Goat anti-Rabbit and actin (green) using Anti- actin Primary Antibody and Star Fluor 488 Goat anti-Mouse.
(D) Immunohistochemical staining on mouse glia of GFAP (blue) using Anti- GFAP Primary Antibody and Star Fluor 645 Goat anti-Rabbit and actin (green) using Anti- actin Primary Antibody and Star Fluor 488 Goat anti-Mouse.
TOPRO-3 counterstain (blue) used to stain nuclei in B and C.
Flow cytometry
Star Fluor secondary antibodies can be used for indirect flow cytometry, where fluorescent conjugated secondary antibodies bind an unconjugated primary.
This approach allows to:
• match the desired probe with any primary antibody
• preserve the active site of the primary antibody
• amplify signal
Flow cytometric analysis of CD-20 staining on EHEB cells. Pseudo-color analysis of the pool of cells stained with anti-CD20 Rabbit Monoclonal Primary Antibody and Star Fluor 645 Goat anti-Rabbit secondary antibody or with secondary antibody only.
Fluorescent Immunoassay
Star Fluor secondary antibodies’ superior brightness and excellent photostability make them a perfect solution to enhance the performance of your assays. They are available in five different colours for multiplex immunoassays, enabling simultaneous analysis of multiple proteins in single samples. They combine the efficiency of multiplexing with the accuracy, sensitivity, reproducibility and stability of fluorescent detection.
Immunoassay for the phosphorylation status of STAT6 using Primary antibody anti-STA6 (green) anti phosphoTyr641 (red).
