STAR BEADS Genomic DNA Extraction kit – CE-IVD
STAR BEADS Genomic DNA Extraction kit – CE-IVD
Rapid isolation of genomic DNA from various biological samples
Overview
STAR BEADS Genomic DNA Extraction Kit provides a fast and efficient purification method to isolate high-quality genomic DNA (gDNA) from biological samples such as whole blood and saliva.
The extracted nucleic acids are highly pure and ready for downstream reactions, including real-time PCR, qRT-PCR, Sanger Sequencing, NGS, and other enzymatic reactions.
For automated scripts and protocols, visit the document section or email to
technical.support@cyanagen.com
Features
• CE-IVDR certified: in compliance with EU regulation 2022
• Magnetic bead-based technology: 50% reduction in plastic use
• Rapid: protocol for manual extraction, automatic extraction time about 60 minutes for 96 and 32 samples
• Easy storage: room temperature
• Easy to use: available in both bottled and prefilled plate format, ready to use by manual procedure or on automatic extractors
• Automatable: Compatible with
– Allsheng Auto-Pure96/Auto-Pure32A/Mini
– Accuris IsoPure™ Mini/96
– Procomcure Phoenix-Pure96/32
– MOLGEN PurePrep 96/32
– BIOER GenePure Pro NPA-32P
– MGI SP-NE32
– BIGFISH BFEX-32
…and many other systems
Ordering Information
Recommended sample input and expected yields from different sample types
Average yield, A260/A280 and A260/A230 were measured using Implen™ NanoPhotometer™ NP80 or Nanodrop2000.
* Saliva stored in OrageneTM collection kit (DNA Genotek).
Isolation of high quality genomic DNA from whole blood
High quality and purity of extracted genomic DNA. gDNA is un-sheared and >48 kb in length. RNA contamination is absent.
Yield and purity of genomic DNA from 200 µL of whole blood. Automated extraction of six blood samples (200 µL).
Effects of freezing and thawing blood samples. EDTA-treated blood was frozen and thawed up to 3 times. Data are represented as mean ±SD (n=16).
Yield and purity of genomic DNA extracted from 200 µL of whole blood collected on citrate, EDTA and heparin tubes. Data are represented as mean ±SD (n=16).
Yield and purity of genomic DNA extracted from different input volumes of whole blood. Graph shows average values from two readings. Data are represented as mean ±SD (n=3).
Performance comparison between STAR BEADS Genomic Kit and kit from Competitor Q. DNA was purified from 200 µL of whole blood. Graph shows average values.
High quality DNA for Next Generation Sequencing
NGS of a custom target gene library starting from genomic DNA isolated with the STAR BEADS Genomic DNA Extraction Kit. DNA was isolated from 200 µL aliquots of frozen whole blood (bl-1,2,3) and saliva (sa_4) using an automated protocol on Allsheng AutoPure 32A. Dual-index paired-end libraries were generated from 150 ng of extracted genomic DNA with the Lotus DNA library prep kit (Integrated DNA Technologies IDT). Target panel hybridisation was performed using the custom xGen Lockdown probe pool (IDT, 224318852, 26 genes) and xGen hybridisation capture of DNA libraries kit (IDT). NGS was performed on the MiSeq™ Sequencing System according to the manufacturer’s instructions on a Nano v.2 flow cell, 300 cycles paired-ends. Reads were properly paired and mapped to unique positions on the human reference genome (hg19) according to a dedicated pipeline (Bonora et al, 2021).
High percent of properly paired reads. Average percentage of properly paired reads is 98,86.
High percent of bases above Q20. Average percentage of bases in which the phred score is above 20 is 99,4%.
High quality DNA for SNP genotyping
SNP genotyping on gDNA isolated with the STAR BEADS Genomic DNA Extraction Kit. Allelic discrimination plots obtained for BCL11A rs1123573 using Taqman SNP Genotyping Method. Red and blue dots represent the homozygous genotypes, green circles represent heterozygous genotypes and squares on the bottom left of the plot are no-template control. No sample gave undetermined result.
Genomic DNA purification from saliva
Yield and purity of genomic DNA from 200 µL of saliva. Three specimens stored in OrageneTM collection kit (DNA Genotek) were extracted with an automated protocol. Data are represented as mean ±SD (n=16)
Order in combination with GreenQuant dsDNA kit
for dsDNA quantification in combination with any fluorometer and any fluorescence microplate reader
dsDNA selectivity and sensitivity of GreenQuant dsDNA kit. Triplicate 10 μL samples of λDNA and yeast RNA were assayed with the GreenQuant dsDNA kit. The Graph was plotted as fluorescence versus the RNA or DNA concentration. Background fluorescence was not subtracted and the y-intercept was set at the blank’s RFU.
Comparison of quantification of gDNA samples using GreenQuant dsDNA kit and Quant-iT™ 1X dsDNA HS Assay Kit (Thermo Fisher, Cod. Q33232). Readings by VICTOR® Nivo™ Multimode Plate Reader, PerkinElmer.
Order information
REQUEST a sample to: sales@cyanagen.com
