GreenQuant ds DNA Kit

GreenQuant ds DNA Kit

High selective fluorescent quantitation of double-stranded DNA 

The GreenQuant dsDNA Kit contains a fluorescent proprietary DNA-binding dye (488nmEx/522nmEm, Fig.1) that allows accurate and sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in a purified sample.

The assay is highly selective for dsDNA over RNA (Fig. 2) and provides a linear range between 0.2 and 200 ng of dsDNA input, corresponding to sample concentrations of 0.1 ng/μL to 100 ng/μL. For more concentrated DNA samples, diluting them with GreenQuant Buffer 1X is recommended. Compared to spectrophotometric DNA quantitation, the assay provides key advantages such as significantly increased sensitivity, high selectivity for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA, and improved contaminant tolerance (protein and carbohydrate molecules). The kit is ideal for quantifying DNA from low-concentration or precious samples and for quantifying DNA for use in sensitive applications such as Next-Generation Sequencing (NGS) or digital PCR.

The GreenQuant dsDNA Kit includes concentrated GreenQuant Dye, dilution buffer 100X, and λ DNA (20 ng/μL) to prepare serial dilutions for the standard curve.

The kit is intended for any fluorometer (as QubitTM Thermo Fisher Scientific) and any fluorescence microplate reader at the appropriate excitation and emission wavelengths. The signal is stable up to 4-6 hours when the samples are protected from light.

The kit is sufficient for 25 or 1000 assays.

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