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Sometimes, at the end of a Western blotting process, no bands or weak bands are on the membrane.The absence of bands, or their faint expression, may be due to several factors.We provide hints to fix the issue for each root cause. • Excessive signal generated. The enzyme in the system depleted the substrate, leading to […]

Sometimes, at the end of Western blotting, the result is a blot with irregular black spots.Several factors may cause Irregular Black Spots.We provide hints to fix each root cause. • Air bubbles are trapped in the membrane. Remove air bubbles by gently rolling a clean pipette or a test tube during sandwich assembly. • Unevenly […]

Sometimes, at the end of a Western blotting process, the result is a dark membrane with a high background. A high membrane background may result from several factors. We provide hints to fix the issue for each root cause. • High concentration of Ab. Further dilute the primary and secondary Abs. Follow the suggested Ab […]

Luminol-based chemiluminescence is a technology that produces light via a chemical reaction between luminol and an oxidizing agent, and is commonly used to detect trace amounts of catalysts, such as iron in blood, or enzymes in labs. In a basic solution, luminol oxidizes, forming an excited-state product that emits blue light (approx. 425 nm) as […]