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NANOPARTICELLE DI SILICE LUMINESCENTI COME MARCATORI PER APPLICAZIONI BIOANALITICHE   Abstract La disponibilità di sorgenti eccitatrici monocromatiche, potenti e a basso costo, e di rilevatori ad altissima efficienza, sta rendendo tecnologie di frontiera, come la rilevazione della singola molecola, sempre più accessibili ad ambiti di ricerca applicativi sfruttando le potenzialità offerte da tecnologie provenienti dalla […]

SYNTHESIS AND ELECTROCHEMILUMINESCENCE OF A RU(BPY)3-LABELED COUPLING ADDUCT PRODUCED ON A SELF-ASSEMBLED MONOLAYER   Abstract In this work, a series of silicon chips containing sputtered gold electrodes were first coated with a selfassembled monolyayer (SAM) of lipoic acid. The same electrodes were then functionalized by using a Ru- (bpy)32+ amine derivative, bis(2,2’-bipyridine)-[4-(4’-methyl-2,2’-bipyridin-4-yl)aminobutyl] ruthenium bis- (hexafluorophosphate). […]

CHEMILUMINESCENT DETECTION SYSTEMS OF HORSERADISH PEROXIDASE EMPLOYING NUCLEOPHILIC ACYLATION CATALYST   Abstract The light output of the peroxidase-catalyzed luminol chemiluminescent oxidation reaction can be greatly increased by incorporating different enhancers. Such an increase is attributed to the preferential oxidation of the enhancer by peroxidase intermediates and the rapid formation of enhancer radicals that, in turn, […]

ELECTROCHEMISTRY AND ELECTROCHEMILUMINESCENCE OF [RU(II)-TRIS(BATHOPHENANTHROLINE-DISULFONATE)]4− IN APROTIC CONDITIONS AND AQUEOUS BUFFERS   Abstract In this work, the electrochemical and ECL properties of tris[1,10-phenanthrolinediyl-4,7-di(benzenesulfonate)] Ru(II) ([Ru(BPS)3]4-) have been addressed in both strictly aprotic conditions and aqueous buffers. A combined theoretical and experimental approach is presented to focus thermodynamics and kinetic effects of electro-generated species possessing highly […]

NEW IODO-ACETAMIDO CYANINES FOR LABELING CYSTEINE THIOL RESIDUES. A STRATEGY FOR EVALUATING PLASMA PROTEINS AND THEIR OXIDO-REDOX STATUS   Abstract Two new iodoacetamide-substituted cyanines, C3NIASO3 and C5NIASO3, were synthesized starting from hemicyanine and were utilized for labeling plasma proteins. Specificity, sensitivity and feasibility for SH residues was tested utilizing an equimolar mixture of standard proteins […]

RU(BPY)3 COVALENTLY DOPED SILICA NANOPARTICLES AS MULTICENTER TUNABLE STRUCTURES FOR ELECTROCHEMILUMINESCENCE AMPLIFICATION   Abstract The electrochemiluminescence (ECL) of doped silica nanoparticles (DSNPs), prepared by a reverse microemulsion method that leads to covalent incorporation of the Ru(bpy)3 2+, was investigated in acetonitrile and aqueous buffers. The emission was produced for the first time by cation-anion direct […]

DEVELOPMENT OF A NEW DEVICE FOR ULTRASENSITIVE ELECTROCHEMILUMINESCENCE MICROSCOPY IMAGING   Abstract Electrochemiluminescence (ECL) is widely used in biosensors and immunoassays thanks to the high sensitivity and specificity of the electrochemically triggered luminescence signal. So far, no applications have been reported on the use of ECL as a probe for ultrasensitive lowlightmicroscopeimaging. This work reports […]

PHOTOTHERMAL SENSITISATION AND THERAPEUTIC PROPERTIES OF A NOVEL FAR-RED ABSORBING CYANINE   Abstract A water-soluble disulfonate cyanine was prepared by chemical synthesis and shown to possess photophysical properties which are particularly favourable for the promotion of photothermally sensitised processes, including a very low (

3-(10’-PHENOTHIAZINYL)PROPANE-1-SULFONATE IS A POTENT ENHANCER OF SOYBEAN PEROXIDASE-INDUCED CHEMILUMINESCENCE   Abstract 3-(10’-Phenothiazinyl)propane-1-sulfonate (SPTZ) was shown to be a potent enhancer of soybean peroxidase (SbP)-induced chemiluminescence. To the best of our knowledge, SPTZ is the first enhancer of SbP to be discovered. Optimal conditions for SbP-catalyzed oxidation of luminol in the presence of SPTZ were determined. […]

NEUTRAL AND DIANIONIC RU(II) BATHOPHENANTHROLINEDISULFONATE COMPLEXES: A ROUTE TO ENHANCE ELECTROCHEMILUMINESCENCE PERFORMANCE IN AQUEOUS MEDIA   Abstract We report the strong enhancement of ECL intensity and duration in neutral and dianionic Ru(II) complexes bearing mixed 2,2′-bipyridine (bpy) and bathophenanthrolinedisulfonate (BPS) ligands. In aqueous conditions, using the tripropylamine-assisted method and applying a constant potential, we observed […]