PCNA

Introduction

Proliferating cell nuclear antigen, PCNA, is a cofactor of DNA polymerase delta, and is expressed in the nuclei of cells during the DNA synthesis phase (S phase) of the cell cycle. PCNA is involved in eukaryotic DNA replication. PCNA is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, S. pombe, S. cerevisiae, K. lactis, E. gossypii, M. grisea, N. crassa, A. thaliana, and rice. Human PCNA with 261 amino acids share 97% identity with rodent homologs and 36% identity with yeast homologs. PCNA is not a good loading control for non-proliferating cells or cells with anti-proliferation treatment.

Properties/Highlights

Tool for normalizing gel loading differences and for Western blot quantification
Se
nsitive and reproducible 

Rabbit

WB, IHC, IF, IP

Hu Ms Rt

1 mg/mL

29 

Western Blot dilution  1:500-1:2000
Immunohistochemistry dilution  1:50-1:200
Immunofluorescence dilution  1:20-1:50
Immunoprecipitation dilution  1:20-1:50

Room Temperature

Store at -20°C for long-term storage (recommended)

Store at +4°C for short-term (1-2 weeks)

Avoid freeze/thaw cycle

Western Blot analysis of extracts of various cell lines,
using PCNA Polyclonal Antibody ABP141

Order guide

Order-No.Unit SizePrice
ABP141,01001 mg/mL260.00

Support Files

Search for CoA or CoC

Related Products