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Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Tool for normalizing gel loading differences and for Western blot quantification |
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Rabbit |
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WB, IHC |
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Hu Rt Ms Mk Chk Hm Sh Xenopus |
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1 mg/mL |
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37 |
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Western Blot dilution 1:5000 - 1:20000 |
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Room Temperature |
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Store at -20°C for long-term storage (recommended) Store at +4°C for short-term (1-2 weeks) Avoid freeze/thaw cycle |
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Western Blot analysis of 293T(1), Rat brain(2), NIH 3T3(3), Sheep Muscle(4), Rabbit testis(5), using GADPH polyclonal antibody ABP136 diluted at 1:20000
Order-No. | Unit Size | Price |
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ABP136,0100 | 1 mg/mL |