IR-BLOT 700 Goat anti-Mouse

Introduction

IR-BLOT secondary antibodies are high quality secondary antibodies optimized for quantitative Western blotting in the infrared region. These antibodies are highly cross-adsorbed, so ensuring extremely low background and very specific signal. The high fluorescence intensity of IR-BLOT secondary antibodies allows a very high sensitivity and an optimal signal to noise ratio, both in the 700 nm and 800 nm channel. In order to provide the best value for money, IR-BLOT secondary antibodies are optimized for multiplex infrared Western blotting and for lower optimal working dilution. IR-BLOT secondary antibodies can be used on instruments with excitation and emission filters in the infrared region.

Properties/Highlights

Highly cross-adsorbed antibodies
High sensitivity and low background
Quantitative Western blotting
Optimized for multicolor IR-Western blotting 

 

678-690 nm (abs-em)

 

Infrared quantitative Western blotting

0.1 mg of fluorescent secondary antibody lyophilized in phosphate buffer saline, pH 7.4. Contains 10 mg/mL BSA as stabilizer and 0.01% sodium azide as preservative, after reconstitution

Refrigerated

Protect from light. Store at +4 °C prior to reconstitution. Once reconstituted, stable for up to 3 months at +4 °C

 IR-BLOT 700 Goat anti-Mouse – Cyanagen

 IRDye® 680RD Goat anti-Mouse – LI-COR 

Fig 1. Fluorescent western blotting for the detection of Vinculin on two-fold serial dilutions of HeLa whole cell lysates (from 5 μg to 78 ng). Imager:
ODISSEY ® CLX – LI-COR

Fig. 2 Multiplex fluorescent western blotting for the detection of vinculin (IR-BLOT 700 Goat anti-Mouse – Cyanagen, red) and HSP90 (IR-BLOT 800 Goat anti-Rabbit – Cyanagen, green) on two-fold serial dilutions of HeLa whole cell lysates (from 5 μg to 78 ng). Imager: ODISSEY ® CLX – LI-COR

Order guide

Order-No.Unit SizePrice
F2H112,S0010,1 mg85.00
F2H112,S0055 x 0,1 mg140.00

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