GREEN STAIN - DNA Loading Dye

GREEN STAIN - DNA Loading Dye

Introduction

Green Stain - DNA Loading Dye contains a bright green fluorescent dye for DNA stain with a very low toxicity and a pre-mixed loading buffer with tracking dyes (blu and xylen) for agarose gels. Green Stain-DNA Loading Dye allows simultaneous staining of DNA and visual tracking of the migration. Green Stain - DNA Loading Dye is easy-to-use: just add it to the sample before pipetting it into the gel wells. Gel visualization can be done with a standard transilluminator (254 nm), or with a laser- or LED-based imaging system selecting the SYBR® Green filter (460-490).

Properties/Highlights

Very low toxicity;

Ready to use kit;

Optimal signal to background ratio;

Simple mix with your DNA sample and load into the gel.

 

DNA staining for agarose gel electrophoresis

Vial A: Green Stain – Dye, Cod. NAGS116,DY

Vial B: Green Stain - Loading buffer, Cod. NAGS116,LB

Refrigerated

Store at – 20°C; after recostitution, store at 4°C for 6 months.

Excitation/emission spectra of Green Stain bound to dsDNA

DNA Ladders and genomic DNA: Green Stain – DNA Loading Dye

A) Genomic DNA.

B) 200 ng of GeneRuler™ 100 bp Plus DNA Ladder (Thermo Scientific). 

C) 200 ng of GeneRuler™ 1 Kb Plus DNA Ladder (Thermo Scientific). 

All the samples were stained with Green Stain –DNA Loading Dye and  electrophoresed on a 1% agarose gel at 100 V for 60 minutes. Gel visualization was done with GE Healthcare "ImageQuant ™ LAS 4000" (460 nm filter), exposition time 2 sec.

Order guide

Order-No.Unit SizePrice
NAGS116,CLK011 x 1 mL40.00
NAGS116,CLK055 x 1 mL175.00

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