PRO-LINER 3-COLOR

Introduction

The PRO-LINER 3-COLOR Protein Marker – Broad range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer.

Properties/Highlights

Ready-to-use: direct loading on gels *
Sharp bands: color-coded bands of similar intensity for easy visualization
Membrane compatibility: colored bands transfer to membranes for western blotting

 

* Do not heat, dilute, or add reducing agent before loading

 

From 5 to 245 kDa in Tris-Glycine buffer

25 kDa (green band) and 75 kDa (red band)

3 or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini gel;

1,5 - 2,5 μL per well for general western blotting transfer;

apply more for a thicker (>1.5 mm) or larger gel;

+4 °C for 3 months, -20 °C for 24 months

 

 

 

Band

Color

TRIS-GLYCINE

BIS-TRIS (MOPS)

BIS-TRIS (MES)

1

Blue

245

235

235

2

Blue

180

170

170

3

Blue

140

130

130

4

Blue

100

93

93

5

Red

75

70

72

6

Blue

60

53

53

7

Blue

45

41

42

8

Blue

35

30

30

9

Green

25

22

23

10

Blue

20

18

18

11

Blue

15

14

14

12

Blue

10

9

10

13

Blue

~5

~3.5

~3.5

 

Guide for Molecular Weight Estimation
Migration patterns and approximate MWs (kDa) of PRO-LINER 3-COLOR Protein Marker – Broad range in different electrophoresis conditions 

Order guide

Order-No.Unit SizeQ.tyPrice
MWM114,00052x250 µL1115.00

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