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RUBY GEL STAIN is a ready to use kit for rapid and sensitive protein staining in 1D and 2D SDS polyacrylamide electrophoresis gels.
The staining procedure is a simple 220 minutes, three step protocol.
It enables optimal visualization and quantitation of proteins with high contrast and the same sensitivity as silver staining without its drawbacks.
Polyacrylamide gel staining is a crucial technique in total protein analysis.
Conventional methods for universal profiling of proteins in gels include Coomassie brilliant blue staining and silver staining. Although Coomassie brilliant blue is an inexpensive reagent, its staining is relatively insensitive and time consuming. Silver staining may be up to 100 times more sensitive than Coomassie brilliant blue staining, but it is quite expensive and requires several laborious and time-sensitive steps. Moreover, it also shows a high degree of protein-to-protein variability and a very poor linearity with protein concentration and poor reproducibility in staining from gel to gel, making it inadequate for comparative studies of protein expression cells.
The recent improvements in fluorescence imagers and fluorescent applications have resulted in greater demand for fluorescent stains. RUBY GEL STAIN provides exceptional fluorescent staining performance with fast and easy procedures. The fluorescent stain involves simple dye-binding mechanisms rather than chemical reactions that alter protein functional groups. Therefore, after staining, it is compatible with protein recovery methods for downstream analysis by mass spectrometry.
The dye has optimal excitation at 302 and 470 nm, with an emission maximum at approximately 610 nm.
RUBY GEL STAIN can be excited with UV-light, UV transilluminator and 405, 445, 473-488 nm laser sources.
IMAGES AND COMPARISON WITH THE MOST USED GEL STAINING REAGENTS
|1D GEL||2D GEL|
|Two-fold dilution series of Precision Plus Protein™ Unstained Bio-Rad marker, separated on Bio-Rad Mini-PROTEAN® TGX™ 12% and stained following RUBY GEL STAIN 220 min protocol. Images acquired by GEHC “ImageQuant™ LAS 4000”, Exc 312 nm, exposure time 1s. Lane 9 is approximately 0.4 ng.||60 µg of Bio-Rad Readyprep™ E.coli protein Sample, separated on Bio-Rad Readyprep™ IPG strips pH 3-10 NL and then on SDS-PAGE (T%8-16) with a Bio-Rad PROTEAN® II XI system. The gel was stained following RUBY GEL STAIN 220 min protocol.
The image was digitalized by a Bio-Rad Molecular Imager FX™ Pro fluorescent imager, PMT Voltage Medium Sample Intensity.
|RUBY GEL STAIN on a 1D gel. Two-fold dilution series of Precision Plus Protein™ Unstained Bio-Rad marker, separated on Bio-Rad Mini-PROTEAN® TGX™ 12%.
Lane 9 is approximately 0.4 ng.