IR FLUORESCENT WESTERN BLOTTING

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IR FLUORESCENT Western Blotting

Introduction

Fluorescent Western blotting is the best option for quantitative and accurate protein expression analyses.

Fluorescence detection in Western blotting allows to obtain reliable and reproducible results and provide a wider dynamic range than other available detection methods.

Fluorescent signal intensity does not vary with time of exposure, and is highly stable: the blots can be stored and reimaged after months without alteration in signal intensity.

It offers the ability to assay multiple targets on the same blot, at the same time, without stripping and reprobing the blot.

IR-BLOT secondary antibodies are high quality secondary antibodies optimized for quantitative Western blotting in the infrared region.

Features

  • Highly cross-adsorbed antibodies

  • High sensitivity and low background

  • Quantitative Western blotting

  • Multiplex Western blotting for simultaneous detection of different proteins

  • Extended signal stability

Fluorescent Western blotting is the best option for quantitative and accurate protein expression analyses.

IR-BLOT secondary antibodies are high quality secondary antibodies optimized for quantitative Western blotting in the infrared region.

IMAGES AND COMPARISONS:

Fig. 1. Fluorescent western blotting for the detection of Vinculin on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng).  Imager: ODISSEY ® CLX – LI-COR

Fig.2. Multiplex fluorescent western blotting for the detection of vinculin (IR-BLOT 700 Goat anti-Mouse  – Cyanagen, red) and HSP90 (IR-BLOT 800 Goat anti-Rabbit  – Cyanagen, green) on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng).  Imager: ODISSEY ® CLX – LI-COR

 

 

Fig.1. Fluorescent western blotting for the detection of HSP90 on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng).  Imager: ODISSEY ® CLX – LI-COR

 

 

Fig.2. Multiplex fluorescent western blotting for the detection of vinculin (IR-BLOT 800 Goat anti-Mouse  – Cyanagen, red) and HSP90 (IR-BLOT 700 Goat anti-Rabbit  – Cyanagen, green) on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng).  Imager: ODISSEY ® CLX – LI-COR

 

 

Fig.1. Fluorescent western blotting for the detection of Vinculin on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng).  Imager: ODISSEY ® CLX – LI-COR

 

 

Fig.2.  Multiplex fluorescent western blotting for the detection of vinculin (IR-BLOT 800 Goat anti-Mouse – Cyanagen, green) and HSP90 (IR-BLOT 700 Goat anti-Rabbit – Cyanagen, red) on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng).  Imager: ODISSEY ® CLX – LI-COR

 

 

Fig.1. Fluorescent western blotting for the detection of HSP90 on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng). Imager: ODISSEY ® CLX – LI-COR

 


 

 

Fig.2. Multiplex fluorescent western blotting for the detection of vinculin (IR-BLOT 700 Goat anti-Mouse – Cyanagen, red) and HSP90 (IR-BLOT 800 Goat anti-Rabbit – Cyanagen, green) on two-fold serial dilutions of HeLa whole cell lysates (from 5 µg to 78 ng). Imager: ODISSEY ® CLX – LI-COR